Figure 3

RT-PCR analyses for the 33.15 tagged mRNA transcripts using internal primers and cDNA from different somatic tissues, gonads and spermatozoa. Transcript IDs are given on the right and names of the tissues on top. Quality and quantity of the cDNA samples were normalized by PCR with β-actin derived primers. Tissue specificities of the transcripts were ascertained on the basis of presence or absence of the amplicons using corresponding cDNA templates which were further confirmed by Real Time PCR and Southern blotting.