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Table 1 Detailed analysis of the spermatozoal mRNA transcripts tagged with consensus of 33.15 repeat loci in buffalo Bubalus bubalis and their homology or similarity with genes across the species#

From: Expressional dynamics of minisatellite 33.15 tagged spermatozoal transcriptome in Bubalus bubalis

Transcript ID Accession no. Size (In bp) Clone ID Description of Blast search Accession no. of similar genes Gene length (bp) Chromo-Somal location Nucleotide position of 33.6 tagged transcripts Score QC (%) E-value Similarity (%)
JS1 EU348479 276 pJSC39 1. Bos taurus chromosome 1 reference assembly (based on Btau 4.0); featuring in this part similar to uncharacterized protein C3orf44 NC007299.3 161106243 1 119054612–119054872 446 94 5e-112 95
     2. Homo sapiens cDNA FLJ30354 fis, clone BRACE2007682 AK054916 1698 --- 590–699 77 39 4e-111 76
     3. Homo sapiens chromosome 19 clone CTD-2292J18 AC090427 109233 19 72209–72318 77 39 4e-111 76
     4. Homo sapiens tetratricopeptide repeat protein 12 (TTC12) gene, alternatively spliced EF445041 64712 --- 10050–10141 55 35 1e-04 74
JS2 EU348480 276 pJSC40 1. Bos taurus chromosome 1 reference assembly (based on Btau 4.0) NC007324.3 53376418 23 277117728–277117989 412 97 3e-113 94
     2. Bovine steroid 21-hydroxylase gene (P-450-c21) gene M11267.1 6601 23 6323–6583 414 97 9e-113 94
     3. Ovis aries cytochrome P-450 steroid 21 hydroxylase (P-450-c21) gene EF382834 3454 20 3009–3272 411 97 1e-111 94
     4. Sheep cytochrome protein (P450C21C) mRNA M92836 2020 --- 1862–2018 263 57 3e-67 96
     5. Bovine microsomal cytochrome P-450(C21) mRNA M12918 2157 23 2005–2157 255 55 7e-65 97
     6. Bos taurus cytochrome P450, subfamily XXI (CYP21A2), mRNA NM_174639 2125 23 2005–2125 203 43 8e-51 97
JS3 EU348481 270 pJSC41 1. Homo sapiens BAC clone RP11-498O22 from 2 AC012506 197303 2 172668–172905 84.2 83 3e-113 69%
JS4 EU348482 267 pJSC42 No homology         
JS5 EU348483 297 pJSC43 1. Bos taurus chromosome 5 reference assembly (based on Btau 4.0) NC_007303 125847759 5 1233822–1234084 422 87 6e-105 92
JS6 EU348484 522 pJSC44 1. Bos taurus chromosome 9 reference assembly (based on Btau 4.0) NC_007307 108145351 9 103122927–103123426 803 98 0.0 94
     2. Human DNA sequence from clone RP1-230L10 on chromosome 6 Contains part of a novel gene AL137005 103679 6 54567–54689 77 23 8e-111 76
JS7 EU348485 517 pJSC45 1. Bos taurus chromosome 29, reference assembly (based on Btau_4.0), NC_007330 51998940 29 30255363–30255634 448 76 2e-123 97
     2. Bos taurus chromosome 16, reference assembly (based on Btau_4.0), NC_007314 77906053 16 45151977–45152179 326 40 9e-87 97
     3. Bos taurus Y Chr NOVECTOR CH240-223B23 (Children's Hospital Oakland Research Institute Bovine BAC Library (male AC220322 194127 Y 25812–25932
29557–29670
117307–117421
200 23 5e-48 96
     4. Bos taurus molybdenum cofactor synthesis 3 (MOCS3), mRNA XM_589014 2797 13 2194–2393 193 21 9e-46 95
     5. Bos taurus T cell receptor gamma cluster 2 (TCRG2) gene AY644518 188109 --- 145614–145734
77004-77124
24056-24175
130049–130169
200 23 3e-50 96
     6. Bos taurus clone p68.3 MHC class I antigen gene, 5' UTR and partial cds AF396777 1169 --- 548–668 195 23 2e-46 95
JS8 EU348486 537 pJSC46 1. Bos taurus chromosome 2, reference assembly (based on Btau_4.0) NC_007300 140800416 2 34260844–34261131 484 98 2e-134 97
     2. Bos taurus chromosome 11, reference assembly NC007309 110171769 11 -- 201 99 3e-49 87
     2. Bos taurus chromosome X, reference assembly NC_007331 88516663 X 51716993–51717467 235 99 1e-59 71
     3. Bos taurus ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), mRNA NM_001101085 3652 13 2472–2652 134 33 2e-30 77
     4. Bos taurus similar to mutant UDP-N-acetylglucosamine 2-epimerase/N-acetylman-nosamine kinase (GNE), mRNA XM_001250216 6177 8 4822–5284 114 80 2e-24 67
     5. Rhinolophus ferrumequinum clone VMRC7-44G7 AC149031 192138 --- 44739–45277 341 91 8e-49 77
     6.Felis catus clone RP86-177C24 AC087421 14'1881 --- 4255–4688 199 86 1e-47 70
     7. Equus caballus subclone 355H03 3.3 MHC class I antigen gene DQ083409 13464 ECA20 2473–3016 187 91 6e-44 68
JS9 EU348487 387 pJSC47 1. Bos taurus chromosome 10, reference assembly (based on Btau_4.0) NC_007308 106383598 10 53535034–53535410 616 98 3e-174 97
     2. Homo sapiens chromosome 15 clone RP11-358M11 map 15q21.3 AC016525 256019 15q21.3 119191–119420 158 59 2e-35 75
JS10 EU348488 398 pJSC48 1. Bos taurus chromosome 2, reference assembly (based on Btau_4.0) NC_007300 140800416 2 89906829–89907043
38557472–38557648
327 98 2e-87 94
95
     2. Bos taurus Y chr BAC CH240-96I24 (Children's Hospital Oakland Research Institute Bovine BAC Library (male) AC213711 186273 --- 149863–150033
117725–117896
29045–29213
282 52 4e-75 96
94
91
     3. Bos taurus Fanconi anemia, complementation group M, mRNA BC149286 1448 --- 1228–1398 282 42 2e-75 96
     4. Bos taurus similar to zinc finger protein 568 (ZNF793), mRNA XM_001254432 5407 18 4744–4914 264 42 5e-70 94
     5. Bos taurus similar to peroxisomal long-chain acyl-coA thioesterase (LOC516537), mRNA XM_594692 2022 10 1603–1773 264 42 5e-70 94
JS11 EU348489 370 pJSC49 1. Bos taurus chromosome 12, reference assembly (based on Btau_4.0 NC_007310 85358539 12 82142726–82143101 513 98 2e-143 90
     2. Bos taurus BAC CH240-10G15 (Children's Hospital Oakland Research Institute Bovine BAC Library (male) AC149774 186916 --- 129128–129350 240 60 2e-60 88
     3. Bos taurus cDNA clone IMAGE:8284348 & CR4-NOT transcription complex, subunit 8 BC119978 2730 --- 545–742 235 53 5e-61 86
     4. O. aries KII-9 gene for hair type II keratin intermediate filament X62509 7371 --- 1823–2030 221 56 2e-54 83
     5. Bos taurus similar to Lysophosphatidic acid receptor Edg-7 (LPA receptor 3) (LPA-3) (EDG7), mRNA XM_612024 2913 3 2138–2356 219 58 7e-54 82
     6. Bos taurus similar to Myotubularin-related protein 2, mRNA BC148076 5057 --- 4447–4665 215 58 9e-53 82
JS12 EU348490 375 pJSC50 1. Bos taurus chromosome 16, reference assembly (based on Btau_4.0) NC_007314 77906053 16 1730212–1730571 563 96 2e-158 94