Table 7 New microsatellite markers developed from the chicken genome assembly (galGal3, http://genome.ucsc.edu/cgi-bin/hgGateway).
Marker name | Position (galGal3 assembly) | Upper primer | Lower primer | PCR annealing T° |
|---|---|---|---|---|
SEQALL0348 | chr1:135392697-135392876 | GCTCAGCACCTCCTCCTC | AGAAAGCAGCCTCACAAAGC | 55°C |
SEQALL0349 | chr1:135543677-135543882 | AGGGTTTCCAAGTGGTGTTG | ACCTTGCCTGAGACTGGTG | 55°C |
SEQALL0350 | chr1:135576325-135576564 | CGACAGATGGTCAAGAATGG | ACACAGTTCTTCGCTGTACG | 55°C |
SEQALL0468 | chr1:64162253-64162451 | TTGCCATTCGAAACATCAAC | GGACTCTGCTGTGCCAAATAC | 50°C |
SEQALL0469 | chr1:64480773-64480997 | TGAGATGATGAATGGCTTGG | ATATGCAGCAGGGCTCATTC | 58°C |
SEQALL0470 | chr1:72300927-72301126 | CACAGGTCCACGAGAAAAGG | TGCAGTCCTTCACATTCTGC | 58°C |
SEQALL0471 | chr1:78494600-78494914 | AGCATCATGACAGTCCAACC | AGGCCAAATTGTCTCACTCG | 58°C |
SEQALL0472 | chr6:24021693-24022070 | ATCATGCGGCAGAAAAAGAG | AAATCCATCAGCCTGACAGC | 50°C |
SEQALL0473 | chr6:24772426-24772628 | GAGAGGGAGGGAGATGAAGG | GAAGAGCTCGTGCAAAAAGC | 58°C |
SEQALL0474 | chr6:25154425-25154640 | TAGGGTTTGGCTGTTGTCTG | CATTTGGAAACCCAGAGCAG | 58°C |
SEQALL0475 | chr15:4463535-4463868 | ACAGGATGCTGCTGCTCAC | AATGTTTCCCTTTCCCAACC | 58°C |
SEQALL0476 | chr15:8651118-8651325 | AGCCAAATCCTGTTTCATGC | TGTCCTTTCTGGGAGAGACG | 58°C |
SEQALL0477 | chr15:9172235-9172553 | CATCCAAGTAACCCCACCTG | TGTAAGGTGTAGGGCGTTGG | 58°C |
SEQALL0478 | chr1:72181285-72181516 | TGGCTTATGGCAACAAAAATC | TGGCCGGTAGTGGATAAAAG | 58°C |
SEQALL0479 | chr1:68536797-68537124 | GATGTTCCTGGCACTTATAGGG | TTTCCCTTTGTTTCTGCATCC | 58°C |
SEQALL0481 | chr1:142664771-142665102 | GCAGCTGCTGTTCCACAAG | GCTCATTGCATTTTTGCTTTC | 58°C |
SEQALL0482 | chr2:88572048-88572376 | GGAAGTGTGTGTTATGGACTGG | CTAGGCTGGAACTGGCAGTAG | 58°C |
SEQALL0483 | chr2:90911596-90911826 | TCTTGGAGATCACGGGAAAG | TGCAACATCAGGACAAGAGG | 58°C |
SEQALL0484 | chr6:5752041-5752395 | CGAGTCTGCTGAATGGACAC | GCGCAATTAATTCAGGAAGG | 58°C |
SEQALL0485 | chr6:6245960-6246304 | GACCACACAGTCACACGTCAG | TCTGGAGGAAGCAATAAGAAGTG | 58°C |
SEQALL0486 | chr6:25680338-25680656 | TTCCCCAGGTGCTCTATCAG | ACCATCGTCTCCTGCCATAG | 58°C |