Table 3 Q-PCR primers with RSq and efficiency values
Gene Name | Clone ID | Primer Sequence | RSq | % Efficiency |
|---|---|---|---|---|
Housekeeping sequence | Â | Â | Â | Â |
Unknown | sb_009_01G07 | F: CTCGGACTCAGCCTGTCTA R: ATAAATCGGACTTCCATTTCCA | 0.982 | 95 |
Aquaporins | Â | Â | Â | Â |
AQP-A | sb_006_02P03 | F: ATGTTGTAGGAGGAAGCGTCA R: ACAAACCGTCGTCTCGGTAG | 0.991 | 115 |
AQP-B | sb_006_05H07 | F: ATTGGTGCTTGGTTGTTGAA R: AATCCTGCTCCAGTGAATCC | 0.996 | 98 |
AQP-C | sb_006_08O08 | F: TACCGTCGCTTCCATGATTA R: CCCTTCACGTAGCTCCAGTT | 0.995 | 105 |
Heat shock proteins | Â | Â | Â | Â |
sHSP | sb_009_11D21 | F: ACTCCACGCCAGCATTTCTTC R: TCCAATTCTGTCCGCATTATTCC | 0.991 | 111 |
HSP70 | sb_009_03K12 | F: CAAGGAGGTGGACAACAACAATC R: CACAATCATTCAGCAGCAATAACAC | 0.994 | 92 |
Antioxidants | Â | Â | Â | Â |
Superoxide dismutase | sb_009_01L22 | F: ACGAGAAGGTTGACGATTTGGG R: ATTCCGCAGCCTAAACGAGAC | 0.998 | 86 |
Catalase | sb_006_03D21 | F: CCCGAGATGGATTTATGAGTCC R: CTAAGTACACAGAAGCCACACC | 0.991 | 183 |
Glutathione-S-transferase | sb_006_04F01 | F: TGCTCCAAGTCGTGCCGTTC R: GATGGCTCGTGACTCGCTTAG | 0.988 | 153 |
Various | Â | Â | Â | Â |
Ferritin | sb_006_04H15 | F: GGAGGTCGTGTCGTTCTTCAG R: CAGTTGTGGGTCACCATTTCG | 0.991 | 94 |
Desaturase | sb_006_09F19 | F: GCTCCTGACCCGTAAACATCC R: CCATGCTGCCAATAAACTTTCACC | 0.996 | 105 |
Trehalose GO annotations | Â | Â | Â | Â |
Trehalose 6 phosphate synthase (1) | sb_006_04P17 | F: TGAATTGGACGATTACGCTGAAG R: AGACTGCCCATTGCTTTGAGG | 1.00 | 86 |
Trehalose 6 phosphate synthase (2) | sb_006_06G01 | F: GGATGGAATTACTGGAGCTTGG R: GTGCTTGATGAGCTGTGAAACC | 0.985 | 110 |
LATS tumour supressor | sb_006_04E02 | F: CGGGACGGACATATCAAACT R: TGAGCAAGGCATCGTACATT | 0.995 | 97 |
Serine/threonine protein kinase 38 | sb_006_04N08 | F: GACTGGTGGAGTCTGGGAGT R: CAGTCCACGCTTTTGAAGAA | 0.992 | 122 |
Protein kinase A cAMP dependant catalytic sub-unit | sb_006_07A12 | F: TCAAAGGTCGAACCTGGACT R: AACCGTACTTTCCCTGCAAC | 0.990 | 100 |
Trehalase precursor | sb_006_01B14 | F: CCGTAGATGGACTTCCTGGT R: TGCCTGTCAGAACACACAAA | 0.985 | 117 |
P70 ribosomal protein S6 kinase | sb_006_01P13 | F: GGGCGAGATGCTAATGAAAT R: CCTTCGTGTGAGCAGTGTCT | 0.991 | 115 |
Putative protein kinase DC2 | sb_006_05C18 | F: ATGGAGAATGGCACTGAGGAC R: CTTAGGCGTCTTTGGTAAACATCC | 0.995 | 99 |
Similar: serine/threonine protein kinase 6 (Aurora family kinase 1) | sb_006_07A19 | F: ACTTTGACATTGGGCGTCTC R: CACCAGGGGCATATTCAAGA | 0.934 | 180 |
Protein kinase C | sb_008_03M09 | F: ACTCCACGATGATGTGTTGTATCC R: TCCAGAACCACTTCCTTGATTGC | 0.995 | 80 |
cAMP dependant protein kinase C1 | sb_009_02D08 | F: CAACGTCATCTACCGTGACC R: AAATACTCCGGTGTCCCACA | 0.988 | 102 |