Figure 2

Map showing positions of oligonucleotide PCR primers for gene AAEL004181 and the positions of introns (white boxes) present in the Aedes aegypti Vectorbase annotation for this gene (see Table 1 for primer sequences, product sizes and conditions used in the study). The black box represents an extension of the gene compared to the annotation in Vectorbase. Primers AAEL004181b, c and i were designed to span these introns produced RT-PCR products identical in size to those produced by genomic PCR, confirming that the introns were in fact mis-annotations; RT-PCR using AAEL004181a extended the 5' end of the exon.