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Table 5 List of oligonucleotide primers used in this study

From: The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements

Primer Sequence Positions (bp) Expected length
(bp)
TvMULE1_F 5'-AAGCGAGCATGAACTGCATCA 229–249 696
TvMULE1_R 5'-TTCCGATCAAGGTCCGGCAATTA 902–924  
TvMULE2_F 5'-GCTGACTGTGCGCTAAACATTGCT 1055–1078 544
TvMULE2_R 5'-GCTCAACAATCTGATTACCTGCCC 1575–1598  
TvMULE3_F 5'-GGGTATCAAAGAACAAGAGTCACC 1,286–1,309 630
TvMULE3_R 5'-TCTCTTTCAGCGGCTGTCCATCTT 1,892–1,915  
TvMULE4_F 5'-GGACAAACTCGCCCATTGTCACTT 8–31 584
TvMULE4_R 5'-TCTTGACAGGTGGATGCTTCGCTA 568–591  
TvMULE4_2F 5'-TTCGCCTTTCTGGGAAGTACTGGT 485–508 520
TvMULE4_2R 3'-GTCACTGGCAAATTCGCGGAATCA 981–1,004  
β tubulin_F 5'-ACACTCCTTCTCAACAAGCTCCGT 692–715 673
β tubulin_R 5'-AGGCTGTTGTGTTGCCGATGAATG 1341–1364