Figure 4

Evidence for specific metazoan genes isolated from Anemonia , but not found in the Nematostella genome. A: PCR results confirming the metazoan origin of MERP, Ferroxidase and Amine-oxidase genes. Amplifications were performed on genomic DNA from ectodermal cells (specific to A. viridis, E), cultured zooxanthellae (specific to Symbiodinium, CZ), or holobiont tissues (both A. viridis and Symbiodinium, H), and water as a negative control (C). Three other specific genes were used to confirm the resulting profiles: S sp EF2 is the nuclear gene for Symbiodinium sp elongation factor 2, S sp psbA is the chloroplast-encoded gene for Symbiodinium sp photosystem II protein D1, and Av EF1 is the nuclear gene for A. viridis elongation factor 1 alpha. B: Amino acids alignment of several MERP sequences from Anemonia viridis (Av) Acropora millepora (Am), Mus musculus (Mm), Homo sapiens (Hs) and Carassius auratus (Ca). Signal peptides are highlighted in yellow.