Real time RT-PCR results of 13 genes. (A) Validation of the Microarray data by real-time RT-PCR analysis of eight representative genes. Expression levels of eight genes were detected in E75, L75, E90 and L90 by real-time RT-PCR (red) and microarray (blue). R represents the Pearson correlation coefficient. (B) real-time RT-PCR results of genes related to vascular development and vascular permeability. The x-axis represents the genes and the y-axis shows the fold change in expression. Different superscript alphanumeric characters indicate a statistically significant difference at p < 0.05.