Figure 9

Gene variant analysis. A to D: Relative expression of CST11 (Cystatin 11 alternate transcript 1), CST11_ Δ (Cystatin 11 alternate transcript 2), Eg627821 (predicted gene, EG627821 alternate transcript 1),Eg627821_ Δ (predicted gene, EG627821 alternate transcript 2) is represented as the % of total amplicon intensity (grey) and corrected microarray values (black) measured in the 12 samples studied. T: Testis, VE: Vas efferens, 0 to 8/9: nine epididymal zones, VD: Vas deferens). On the top: image of agarose gels used for quantification. E: Comparison of nucleotidic sequences of Sus Scrofa CST11 alternate transcripts showing the primer positions used for amplification. Hyphens represent gaps introduced for optimal alignment. Numbers are residue numbers. Accession numbers for CST11 and CST11 Δ are respectively scac0028.a.13 and scac0036.l.15. F: Amino-acid sequences comparison between Sus Scrofa and Homo Sapiens CST11 isoforms showing that they have a different deletions. Amino acid residues that are identical between human and pig are shaded. Residues shown to belong to the cystatin domain by Pfam analyses are boxed and those characteristics of this domain are in bold. Accession numbers for hCST11, hCST11Δ are respectively NP_570612 and NP_543020. Amino acid sequences for sCST11 and sCST11_Δ are deduced from scac0028.a.13 and scac0036.l.15 respectively. Hyphens represent gaps introduced for optimal alignment. G: Comparison of nucleotide acid sequences of Sus Scrofa Eg627821 alternate transcripts showing that both sequence have a different deletion. Arrows show the hybridization site of primers used for amplification. Accession numbers for Eg627821 and Eg627821 _Δ are respectively BX924253 and BX922624. Hyphens represent gaps introduced for optimal alignment.