qRT-PCR confirmation of relative transcript abundances for microarray-identified, significantly regulated genes. Mean log2 ratios (stage/reference) for selected genes in selected stages determined by qRT-PCR and microarray analyses. Gene ID's are official gene symbols (prefix Tc00.1047053 is truncated) assigned to annotated genes in the reference CL Brener T. cruzi genome. Error bars are standard deviations for three biological replicates for both the microarray and qRT-PCR data. The qRT-PCR and microarray standard deviations reflect biological, not technical variation. A) trypomastigotes B) epimastigotes C) amastigotes.