Integrating microarray analysis and the soybean genome to understand the soybeans iron deficiency response

BMC Genomics

Table 4 Semi Quantitative Real Time PCR Results

Affymetrix Probe Set	Annotation	Conditions of Differential Expression	Differential Expression in Microarray	Diff Express in sqRT-PCR
Gma.13296.3.S1_at	Lipid Transfer	CSSvCSD	190.34	2.62
Gma.17724.3.S1_at	GDSL motif	CSSvCSD	6.26	8.46
Gma.17825.1.A1_at	GDSL motif	CSSvCSD	4.69	10.26
GmaAffx.89896.1.S1_at	Heat Shock Protein	CSSvCSD	23.45	2.04
GmaAffx.93268.1.S1_at	Heat Shock Protein	CSSvCSD	19.91	1.36
GmaAffx.51733.1.A1_at	Ribonuclease T2	CSSvCSD	-13.35	-2.98
GmaAffx.88242.1.S1_at	SnRNP protein	CSSvCSD	3.58	11.18
Gma.16500.1.S1_at	Lipoxygenase	CSSvCSD	11.36	16.9
Gma.3705.1.S1_at	Nitrate Reductase	CSDvICSD	-3.53	-1.101
Gma.9609.1.S1_at	Reductase	CSDvICSD	2.26	2.23
GmaAffx.36066.1.S1_at	Replication Factor	CSDvICSD	2.63	3.58

Details of sqRT-PCR experiments. Affymetrix Probe set denotes the differentially expressed sequence for which primers were designed. Conditions of Differential Expression represent the genotype and growth conditions in the comparison where the sequence was differentially expressed (CSS – Clark Shoots Iron Suficient, CSD – Clark Shoots Iron Deficient, ICSD – IsoClark Shoots Iron Deficient). Differential Expression in Microarray, the change in gene expression for the sequence from the microarray (in fold change). Differential Expression in sqRT-PCR, the change in gene expression for the sequence seen in the sqRT-PCR experiment (in fold change).

ISSN: 1471-2164