Table 8 Combinations of primers used in quantitative PCR assays.
Gene name | Gene function | Primer sequences |
|---|---|---|
18S | Ribosomal protein | F: CTGGTTAATTCCGATAACGAACGAGACTCTA |
| Â | Â | R: TGCTCAATCTCGTGTGGCTAAACGCCACTTG |
Hemocyte defensin | Immune system | F: ACAAATGTAACAGGCATTGTAGGAGCAT |
| Â | Â | R: CATGTGCATCTTCGGTAAAAAGTCCA |
Big defensin | Immune system | F: ATGGACACTAGGAAAGTCTACTGTGTGCT |
| Â | Â | R: ACAAGTGCAACCCAGACCCAAGGTGA |
Lysozyme | Immune system | F: ATAACGAAAGACCAAGCTCGTGCTCT |
| Â | Â | R: GTTTTGGGTCCTAGATCTCCCCTGTA |
C1q – TNF related protein | Immune system | F: ATGCAAGTCAGTGCCGTGATACACCCAGA |
| Â | Â | R: AATAAAGCGCCACTGAAAGTTGTTCCATG |
TRAF-6 | Immune system | F: GAACTAGCAAACAGGAATTGGGAGGCGCT |
| Â | Â | R:GTCAAGTGATGGCTCATCTTGGATGCTGC |
Toll-like receptor | Immune system | F: GTAACAAATTTCACTCTGGCCGCTGACGC |
| Â | Â | R: TAGCTGAAATCCAACGACTGCACCCGTAA |
RACK-1 | Cell communication | F: CCTAACAGATACTGGCTGTGTGCTGC |
| Â | Â | R: GTCTGTCCATCTGCGGACCATGCAAG |
Peroxisome proliferator-activated receptor | Cell communication | F: CATAGCCAATTCCATACCCCTGGCCA |
| Â | Â | R: AGTTGGCATCGCCACTGTCGCTGCTC |
HSP70 | Stress response | F: AATGACAAAGGCCGTCTCAGCAAGGA |
| Â | Â | R: TCTAACCAACTGATGACCTCGCTACA |
Stress-induced protein STI | Stress response | F: GAAGCTGTTGAACAAGCCAAGAGTGGAGC |
| Â | Â | R: GTCTCTTGAATTCGGGGATCTTGAGCTGC |
Ferritin | Iron transport | F: ATGTCTGTTTCACGACCTCGACAGAA |
| Â | Â | R: AGTTTCTCGGCATGCTCACGTTCCTC |
Metallothionein | Detoxification | F: ACCAGTGATGGTGGCTGCAGGTGTGG |
| Â | Â | R: TTACACGAACAGCCACTATCACACTG |
Actin | Cytoskeleton | F: ATTGTGATGGACTCTGGTGATGGTGT |
| Â | Â | R: TCTCTAACAATTTCTCTCTCAGCCGTTGT |
Transcription factor AP-1 | Transcription | F: AGAAAACTTGAAAGAATTGCGCGACT |
| Â | Â | R: TGTGACATCATTATCTGGCACCCACT |
Elongation factor beta | Transcription | F: CCTTGGGATGATGAAACAGATATGGC |
| Â | Â | R: CTAATCTTGGCATCTTCTATAACAGC |
NADH sub-unit IV | Mitochondrial respiration | F: CCGTGGGATTTAGGGAGGGATAATATGCT |
| Â | Â | R: ACTCCAGTTAACAACATTGATCCCCTCAA |
Senescence associated protein | unknown | F: AACCTGTCTCACGACGGTCTAAGCCCAGC |
| Â | Â | R: TTACCACAGGGATAACTGGCTTGTGG |
Cytochrome P450 like TBP | unknown | F: GTCTGGAAAACGGCCACAAGGCACCT |
| Â | Â | R: TTATACAAGGTAACCGGCTTGGACGC |