Figure 2

Representative two-dimensional maps marked altered proteins. Representative two-dimensional maps of a pair of OSCC cancer tissue and precancerous oral leukoplakia tissue from the same patient. The proteins were separated on a pH 3–10 nonlinear IPG strip, followed by a 12% SDS-polyacrylamide gel, as stated under Methods. The gel was Coomassie-blue stained and the spots were analyzed by ESI-Q-TOF-LC-MS/MS. Arrows indicate identified protein spots significantly and consistently altered between carcinoma tissue and control tissue. 11 boxed proteins were selected as examples showing the consistent expression changes in enlarged form in additional file 1.