Figure 6
Organization of dysregulated protein genes into common pathways in the transformation process from precancerous oral leukoplakia to OSCC by Pathway Studio. Proteins with their SWISS-PROT number were loaded into Pathway Studio software http://www.ariadnegenomics.com/, and analyzed using the Pathway Studio program with the ResNet 2.5 database. (A) 85 protein genes with a high confidence index of interactions were included in the pathway layout graph. (B) The indirect interaction of eight proteins with PA28 a and b (gene name: PSME1 and 2) as reported in the cancer-related literature. (C) The key proteins in MHC antigen presenting pathway, among which PA28, proteosome, HSP70 and HSP90 have been detected up-regulated in our study. Each node represents either a protein entity or a control mechanism of the interaction. Connecting lines between the protein symbols indicate interactions; different types of interactions are denoted by symbols on the lines. Green square indicates regulation; purple square, binding; blue square, expression; orange circle, protein modification; red diamond, metabolism; green circle, promoter binding; yellow triangle, transport; "+" in gray circle, positive effect; and "-" in gray circle, negative effect.