Figure 7

Validation of PA28 in OSCC tissues and cells relative to control oral leukoplakia tissue and normal keratinocytes. (A, B, C) The mRNA expression evaluated by real-time PCR in seven OSCC cell lines when compared to the oral keratinocytes. The mRNA level of the three PA28 homologs, a, b and g were shown in A, B, and C, respectively. Except PA28 a in KB and Tca8113 cell lines, the mRNA expression in OSCC cells were reproducibly increased. (D) Crops of images of quantitative measurement of PA 28 g protein in 3 pairs of OSCC tissues (Ca) and oral leukoplakia tissues (control) by Western blotting analysis using PA 28 g-specific antibody. (E) The mRNA expression evaluated by real-time PCR in 4 pairs of OSCC tissues and oral leukoplakia tissues.