Generation, annotation, and analysis of ESTs from midgut tissue of adult female Anopheles stephensi mosquitoes

BMC Genomics

Table 1 Summary of ESTs generated from adult female A. stephensi midgut tissue.

Library feature	SF library	BF library	Combined
Total ESTs Sequenced (High quality)^†	7061	8306	15367
Number of Contigs	822	591	1319^‡
Number of ESTs in Contigs	5243	6951	12740
Number of Singlets	1818	1355	2627
Number of UTs^§	2640	1946	3946
Average length of ESTs	310.69	448.90	380^¤
Average length of Contigs	492.78	651.26	678.96^$
Average %GC	45.70	52.06	48.88

^†A high quality sequence is obtained by phred using quality value ≥ 20 for a stretch 100 bases. ^‡Obtained on assembling ESTs from SF and BF libraries. ^§ UTs are calculated as sum of number of contigs and singlets. ^¤Obtained using all the ESTs together. ^$Calculated using contigs obtained in common assembly of ESTs from both the libraries.

ISSN: 1471-2164