Figure 1
Global tendency of AFAS probes in normal tissues. (A) Mean signal intensities of AFAS probes were compared between sets of known NAT-associated genes (including imprinted genes) and negative controls. Negative controls with no cDNA, EST, and CAGE tags in their antisense orientation were randomly picked from NCBI RefSeq. (B) Nuclear enrichment (grey bars) was measured by log2(Snucleus/Swhole-cell), where Snucleus and Swhole-cell denote signals from nuclear fractions and from whole cells (NIH3T3), respectively. Cytoplasmic enrichment (open bars) was calculated by log2(Scytoplasm/Swhole-cell), where Scytoplasm denotes signals from the cytoplasmic fraction. All signal intensities were obtained from the experiments by using random-primed samples. (C) The sum of Z-scores for every relative position is indicated. For each tissue, the positional preference of NAT expression was measured by Z-scores calculated from the median signal values of every position. Expression data were obtained from random-primed samples.