Genome wide transcriptome analysis identifies new markers for CD16+ and CD16- monocyte (Mo) subsets. (A) Total RNA from matched CD16+ and CD16- Mo samples isolated from 4 different healthy donors were reverse transcribed and hybridized on GeneChip® Human Genome U133 Plus 2.0 Arrays (Affymetrix). Statistical analyses using one way ANOVA was performed to identify differentially expressed genes (p < 0.05). Graph depicts the number of probe sets shared or differentially expressed between CD16+ and CD16- Mo. (B) Graph depicts the fold change expression of probe sets differentially expressed in CD16+ versus CD16- Mo. (C-D) Hierarchical clustering analysis based on c-fuzzy means separated the 8 samples in 2 groups that perfectly matched CD16+ and CD16- Mo; heat maps were generated using differentially expressed genes (>2-fold). Red and green signify increased and decreased gene expression, respectively.