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Table 4 RT-PCR analysis of genes selected form the microarray profile.

From: Hypoxic transcription gene profiles under the modulation of nitric oxide in nuclear run on-microarray and proteomics

  

RAW

Primary Macrophages

Symbol

Ac

Hyp

NO

Hyp

NO

Vegf1

NM_009505.2

5.7 ± 0.27

2.0 ± 0.14

5.5 ± 0.84

3.0 ± 0.46

Ddit4

NM_029083.1

16.6 ± 0.58

3.6 ± 0.53

2.4 ± 0.37

1.4 ± 0.30

Sesn2

NM_144907.1

2.0 ± 0.47

4.2 ± 0.33

2.5 ± 0.64

3.6 ± 0.70

Bnip3

NM_009760.2

24.1 ± 0.42

2.3 ± 0.40

3.6 ± 0.57

2.2 ± 0.56

Trib3

NM_144554.1

3.0 ± 0.51

3.4 ± 0.61

1.5 ± 0.32

1.7 ± 0.35

Atf3

NM_007498.2

4.6 ± 0.54

1.3 ± 0.27

0.9 ± 0.35

0.9 ± 0.31

Frat2

NM_177603.1

2.0 ± 0.45

1.3 ± 0.43

2.1 ± 0.63

1.6 ± 0.51

Cdkn1a

NM_007669.2

3.6 ± 0.61

2.3 ± 0.28

5.3 ± 1.33

4.2 ± 1.30

Scd1

NM_009127.2

1.3 ± 0.20

0.9 ± 0.60

1.0 ± 0.30

1.4 ± 0.54

  1. RAW cells were subjected to hypoxia (1% O2) or NO (0.5 mM DETA-NO) for 6 h and primary macrophages for 12 h. Whole cell mRNA was isolated and analyzed by qRT-PCR. Expression changes of 9 genes were evaluated in relation to the values obtained in parallel for 16S ribosomal protein obtained from 4 individual experiments. Hyp = hypoxic treatment; NO = nitric oxide treatment.
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BMC Genomics

ISSN: 1471-2164

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