Figure 5

Scanning deletion analyses WNT4 promoters in different cell lines. The top panel illustrates the tammar/human WNT4 gene structure. The left side of the diagram illustrates the constructs consisting of the different promoter regions fused upstream of a luciferase reporter, and the right illustrates the relative luciferase activity derived from the constructs using tammar kidney cells (blue), tammar lung cells (red) and human HEK293T cells (black). (A) The -530/+70 fragment in tammar WNT4 proximal promoter showed the maximum activity in the tammar kidney cell line and HEK293T cell line while the -980/+70 fragment showed the maximum activity in the tammar lung cell line; (B) the -781/+102 fragment displayed the maximum activity in all of three cell lines.