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Table 1 Primers designed for promoter analysis

From: Comparative analysis of the mammalian WNT4 promoter

Primers

Sequence(5'→ 3')

function

p hWNT4F1

AAGGTACCGACAGGACAGAGGCAGAGGT

with hRL, 1968 bp

p hWNT4F2

AAGGTACCCACCATCCCAGCACCCAA

with hRL, 1608 bp

p hWNT4F3

AAGGTACCGGAAGGCTGTAGGGAGGTG

with hRL, 895 bp

p hWNT4F4

AAGGTACCCGGCGCTGACAGTCTGGT

with hRL, 255 bp

p hWNT4RL

ATCTCGAGGAAGACGGCGAAGACGAG

reverse primer

p hWNT4 FD

AAGGTACCCAGGACAGAGGCAGAGGTAGG

with hRD, 1061 bp

p hWNT4 RD

ATCTCGAGTTGGGCAGCTTGGGACAG

reverse primer

p tWNT4F1

GAGGTACCGAAGTAACTCAAAAAGGA

with tRL, 2839 bp

p tWNT4F2

ACGGTACCCAGAGCAATCAGGGAAGG

with tRL, 1998 bp

p tWNT4F3

ACGGTACCCCCAGAGCGAATAAGTGA

with tRL, 994 bp

p tWNT4F4

ACGGTACCTGGCCAGCTCCGAGG

with tRL, 616 bp

p tWNT4F5

ACGGTACCCAGCGCAGAGACCAGGGA

with tRL, 315 bp

p tWNT4RL

AACTCGAGCGAGAAGACGGCGAAGAC

reverse primer

p tWNT4 FD

ATGGTACCGCACCTACTATGTCCCAAGC

with tRD, 2168 bp

p tWNT4 RD

TTCTCGAGCTTCCCCTTCCTGCTCCC

reverse primer

p hWNT4F

TGAGTCAAAGCGTGTAAAGC

Human total

p hWNT4R

CTGAGAAGACGGCGAAGA

promoter regions

E1F

CGGGCGGCACGATGAGT

Screening

E1R

CCAGTTGCTGGCGGCTG

exon1

tWNT4F

GAAACCGACGGTGGAAC

Checking

tWNT4R

AGGAGATGGCATAGACGAA

tWNT4 expression (102 bp)

hWNT4F

TCTGACAACATCGCCTACGG

Checking

hWNT4R

GGCACTTGCATTCCACCC

hWNT4 expression (160 bp)

  1. p: promoter; h: human; t: tammar; F: forward primer; R: reverse primer; D: deletion
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BMC Genomics

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