Figure 1

Donor sites used for the remobilization screen. A: The SqET33 donor site (Tol2 transposon insertion in the 3' UTR of zic6). B: The SqET33-E20 donor site (Tol2 transposon insertion 4.2 kb upstream of zgc:66340). The green arrow shows EGFP; krt4 is a minimal promoter; orange arrows represent the 5' and 3' ends of Tol2; coding exons are depicted as blue boxes; and UTRs are represented as open boxes. C: SqET33-E20 F₀ founder fish was outcrossed to wild type and DNA isolated from GFP-negative embryos (negative control, C), GFP-positive embryos showing a SqET33 expression pattern of GFP (donor-type, D), and GFP-positive embryos showing a new expression pattern distinct from the donor one (N), were used for Southern blot hybridization. SqET33, DNA from the donor line. D: A scheme of the Tol2 remobilization screen.