Flowchart of the in silico EST analyses. Chromatograms represent 5'EST end-sequences from four cDNA libraries of fruits with different post harvest treatments. Sequences were filtered, resulting in 41,519 ESTs that were used for assembling contigs. Assemblies generated 4,169 contigs and 6,661 singletons, resulting in a total of 10,830 Unigenes. The singletons were subsequently filtered for low complexity. The consensus sequence of each contig was further analyzed using EuGène'Hom to determine the number of full-length cDNAs. Using this analysis, 1,723 full-length cDNAs were identified (614 full-length cDNAs and 1,109 putative full-length cDNAs). Additionally, the number of ESTs in each contig was analyzed statistically to determine if the gene (s) that represent these contig are differentially expressed under the conditions analyzed .