Figure 3

Western blots for analysis of secretion efficiency. White and black arrows indicate the cell lysate and supernatants fractions, respectively. Grey arrows indicate mature NucA. In addition to the results for eight constructs with signal peptides, the gels shows results for a construct driving cytoplasmic production of NucA (pNuc-cyt), a construct without the nucA gene (pSIP403, a construct for intracellular expression of gusA; [3]; only the cell lysate is shown for this construct) and a sample of pure mature NucA. For all the culture-derived samples, the sample size corresponded to 16 μl of the original culture harvested at an OD₆₀₀ of approximately 1.7.