Figure 6From: Genome-wide expression profiling of in vivo- derived bloodstream parasite stages and dynamic analysis of mRNA alterations during synchronous differentiation in Trypanosoma bruceiIPC expression in different life cycle stages. Lipids extracted from stumpy (Panel A) and monomorphic slender (Panel B) T.brucei, were analyzed by ES-MS/MS for GPIno phospholipids by parent-ion scanning of the m/z 241 as described in experimental procedures. Peak assignments are based on MS/MS daughter ion spectra and comparisons to previous experiments on whole cell extracts. Panel C shows the relative expression of each member of the TbSLS gene family (TbSLS1, Tb9.211.1030; TbSLS2, Tb9.211.1020; TbSLS3, Tb9.211.1010; TbSLS4, Tb9.211.1000) during the differentiation between slender and stumpy forms and at each point during differentiation from stumpy to procyclic forms. TbSLS1 is significantly up-regulated during these transitions.Back to article page