Temporal expression profile of miRNAs obtained with the whole-life test. (A) Specific miRNAs were up-regulated or down-regulated over the entire life cycle of the silkworm. (B) Comparison of the expression profiles of miR-275 and miR-34b during the life cycle of silkworm. (C) Northern blotting analysis of miRNAs during the silkworm life cycle. 5srRNA and U6 were used as the loading controls.