Figure 3

Identification of plates with significant numbers of putative inconsistent BAC clones. A. Plot of plate numbers of the BACs in putative reciprocal pairs plotted against one another. B. Plot of the proportion of consistent BACs in the unpaired group vs. the proportion of consistent BACs in the tail-to-tail group on a plate by plate basis. Numbers indicate plates with large numbers of inconsistent BACs. Plates without any tail-to-tail BACs were excluded from the plot. C. Diagrammatic representations of 384 well plates, CH240-15 and CH240-227. Correct BACs are indicated by black circles, consistent BACs are indicated by blue circles, putative non-consistent BACs are indicated by red circles and inconsistent BACs that have been sequenced are indicated by black rings around the red circle.