Characterization of the oxidative stress stimulon and PerR regulon of Campylobacter jejuni

BMC Genomics

Table 1 Oxidative stress sensitivity of C. jejuni NCTC 11168 mutants containing mutations of perR, fur, ahpC, katA, or sodB genes, and the corresponding complemented strains determined by disk diffusion assays

Strain	H₂O₂	Cumene hydroperoxide	Menadione
C. jejuni NCTC 11168	19.08 ± 0.19	24.50 ± 0.22	29.39 ± 0.53
ΔperR	6.00 ± 0.00	20.93 ± 0.52	33.90 ± 1.98
ΔperR + perR	17.61 ± 0.15	21.89 ± 0.40	30.28 ± 0.24
Δfur	16.73 ± 0.32	27.53 ± 0.64	31.60 ± 1.55
Δfur + fur	20.00 ± 0.25	25.23 ± 0.99	31.08 ± 1.37
ΔperR Δfur	7.89 ± 0.11	22.28 ± 0.36	35.56 ± 0.20
ΔahpC	17.23 ± 0.50	34.87 ± 0.79	29.05 ± 1.81
ΔahpC + ahpC	18.45 ± 0.10	25.40 ± 0.34	28.38 ± 0.39
ΔkatA	28.85 ± 0.87	26.43 ± 0.47	29.85 ± 1.39
ΔkatA + katA	10.83 ± 1.24	23.83 ± 0.17	31.00 ± 1.20
ΔsodB	22.10 ± 0.35	30.75 ± 3.53	38.28 ± 1.96
ΔsodB + sodB	21.78 ± 0.33	25.40 ± 0.17	37.50 ± 2.62

The data represent the diameters (in millimeters) of the clear zones. Data shown are the means ± standard errors of at least triplicate experiments. The diameter of the disk is 6 mm, and each disk contained 10 μL of H₂O₂ (3%), cumene hydroperoxide (3% in DMSO), or menadione sodium bisulfite (90 mM in H₂O).

ISSN: 1471-2164