Glycogenome expression dynamics during mouse C2C12 myoblast differentiation suggests a sequential reorganization of membrane glycoconjugates

Table 1 Data summary of Mus musculus glycogene expression during C2C12 differentiation.

Protein function	Number of known genes¹	Number of analyzed genes²	Number of expressed genes³	Genes with 2× up- or down-regulation⁴	Genes with 4× up- or down-regulation⁴
Glycosyltransferase	209	146 (70%)	108 (74%)	37 (34%)	12 (11%)
Glycosidase	75	53 (71%)	47 (89%)	8 (17%)	2 (4%)
Sugar carrier	34	26 (76%)	19 (73%)	7 (37%)	3 (16%)
Translocase	2	2 (100%)	2 (100%)	0 (0%)	0 (0%)
Sugar metabolism	28	24 (86%)	23 (96%)	4 (17%)	0 (0%)
Lectin	193	102 (53%)	58 (57%)	29 (50%)	15 (26%)
Sulfotransferase	53	22 (42%)	19 (86%)	10 (53%)	5 (26%)
Total	594	375 (63%)	276 (74%)	95 (34%)	37 (10%)

¹The number of known genes was determined in the MGI database [61] according to their functions.
²The number of analyzed genes, except for the sulfotransferase family, corresponded to assays available from the manufacturer. For sulfotransferase genes, 22 probes were chosen from the 35 available. Values in brackets indicate percentages of analyzed genes compared to known genes.
³Number of expressed genes corresponded to significantly expressed genes for at least one kinetic point according to their mRNA quantification by qRT-PCR (Ct<33). Values in brackets indicate percentages of expressed genes compared to analyzed genes.
⁴Up- and down-regulations correspond to variations in transcript quantity with a minimum factor of 2 or 4. Values in brackets correspond to percentages of deregulated genes compared to expressed genes.

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