Figure 4

Formation of the 4632419I22Rik gene in rodents. (A) Schematic representation pre and post insertion of a 4632419I22Rik ancestral TE with locations of amplification primers (red arrows) used to screen for the presence or absence of the 4632419I22Rik gene in mammalian species and CpG island (green bars). Non-coding exons of 4632419I22Rik and neighbouring genes Brip1 and Ints2 are denoted by narrow boxes and coding exons by larger shaded boxes with the direction of transcription indicated by arrowheads. (B) Molecular phylogenetic analysis of the domestication of 4632419I22Rik in rodents and a summary of 4632419I22Rik screening assay. (+) denotes the presence of the 4632419I22Rik gene and (-) denotes the absence of this gene within specific species. Phylogenetic tree of rodent lineage is displayed.