Table 1 Real-time qPCR analyses.
Genbank accessions, target | Primer sequence from 5' to 3' | PCR efficiency |
|---|---|---|
CA366296, Src kinase associated phosphoprotein | F GAGGTGCTCCCAGAGGATGACA R CAGTCCCACAAGCCCTGGTAGT | 2.0 |
CA349943, C-type mannose-binding lectin | F TCCATTGCACTGGGCGATGC R CACTGCTTCCACCTGAGCCTCA | 1.667 |
CA361395, Leukocyte antigen CD37 | F TGCTGAGACAAGCTTCTTCATGCC R CGACATCGTAGCACTTCCACCAAT | 1.696 |
CA364370, CD40 | F CTGTAAACTGCACCCATACTGCGAC R ATGGGCTGAGGCTTGTCTTGTTC | 1.716 |
CU068239, Leukocyte cell-derived chemotaxin 2 | F CTGTGTTGTCAGAGTGCGAGATGGT R TACACACAATGTCCAGGCCCTGA | 1.899 |
CA369467, 5-lipoxygenase activating protein | F TCTGAGTCATGCTGTCCGTAGTGGT R CCTCCCTCTCTACCTTCGTTGCAAA | 1.751 |
CA366162, Alpha-1-antiproteinase-like protein | F CCACAAGGCTGTGCTGAGCGTA R TGAGCATGATGGTGTCTGGGAGAG | 1.702 |
CA366315, YY1 transcription factor | F AAAGAAGACGACGCGCCCAG R GGTGTGGAGATGCTTCCTCATCG | 1.893 |
CF752495, Jun B | F CATCAGAAGTCGGCTCGCTGAA R GGTGTCGGTGTGGTAGTGATGACA | 1.743 |
CA341859, NFkB1 | F CAGCGTCCTACCAGGCTAAAGAGAT R GCTGTTCGATCCATCCGCACTAT | 1.685 |
CA343143, NFkB inhibitor | F TGGTAACCTTGTGAAGGAGCTGGA R GCTCAGCATGTTCTGTGGCTTCAT | 2.00 |
CA361415, Jun C | F CAGCATGACACTGAACCTGGCTGA R GCAAGTTTGAGGAGCTGCACATCC | 2.00 |
CA345853, Plasma glutathione peroxidase precursor | F CCTTCCAGTACCTGGAGTTGAATGC R CTCATGATTGTCTCCTGGCTCCTGT | 1.904 |
CF753103, Glutathione peroxidase-gastrointestinal | F TGTACCTCAAGGAGAAGCTGCCGT R ATTAAGGCCATGGGATCGTCGC | 2.00 |
CA363120, Heme oxygenase | F TGGGTCTGACCTGGGTCCTCTCAT R GAGGGTGGTTTCAGCGTTGAGC | 1.664 |
CA368533, Vitronectin | F AAGCCCTTCGACGCCTTCCT R CCTCTGATGCCCCACTTGTCGTAG | 2.00 |
CA382259, Complement component 4 binding protein | F TGGTGGAGTATCAGTGTGACAGGCA R GGTGGATTTGGCTCAAACTGTCCT | 2.00 |
CA364804, Complement component 5-2 | F AGAACTCTTCCGAGTTGGCATGGT R AGTGATGCTGGGATCCATCTCTGA | 1.952 |
CA366393, Mannan-binding lectin serine peptidase | F TCAGGTGCTGACGGAGAGGTCA R GCACTCTGAATCCCTCTGGTAGGAG | 1.971 |
CA387557, Complement component 1Q binding | F CGGTCTCTCTGGATGATGAGCCATA R CCACATCCACACGACACAGGAGTA | 2.00 |
CA376069, Cytochrome P450 3A27 | F CCAACCTGCTGAACGGAATGAA R AGAACTCCTTCACTTCGATGGCCT | 2.00 |
CA378743, Fibronectin | F GCATGTCTGAGACGGGCTTCAA R AGTCACATCGGAAGTGTCCACTGC | 2.00 |
CA384134, G1/S-specific cyclin D2 | F CATCAGACCACAGGAGTTGCTGGA R AAGTCATTTGGAGTGACAGCCGC | 2.00 |
CA363965, Liver bile salt export pump | F TACGACACCAACGTAGGTTCCCAGG R GGATCTTAGGGTCGCGGATGATC | 1.686 |
CA385588, CREB binding protein | F CTCCAGCCCAGGCCAACTCC R GGCCAGGCAGGTGAGCTCCT | 2.00 |
X64214, A. salmonicida genomic DNA fragment | F GTTTACCACGTAATCTGAATTGTTCTTTTC R ATTGCTTATCGAGGCAGCCAAC | 1.890 |
AJ427629, 18S rRNA | F GCCCTATCAACTTTCGATGGTAC R TTTGGATGTGGTAGCCGTTTCTC | 2.00 |