Figure 2

Angiogenesis-related gene expression in scNT-N and scNT-MUC. Real-time RT-PCR was carried out using primers that were specific for VEGF, Ang-1 and Ang-2, and their cognate receptors VEGFR-1, VEGFR-2 and Tie-2, respectively. Real-time RT-PCR was independently performed in triplicate for different samples and the data were expressed as the mean value of gene expression measured in individual scNT-N and scN-MUC. Gene expression levels were normalized to tubulin gene expression, and are expressed relative to scNT, which was set as 100 (%). * p < 0.05, ** P < 0.01, *** P < 0.005, **** P < 0.001