Figure 4

Nucleotide and deduced amino acid sequences and the genomic structure of human TNFRSF1A beta. (A) Nucleotide and deduced amino acid sequences of TNFRSF1A beta. The PCR amplified sequences (upper case) and 3' prolonged untranslated region (lower case) are indicated. The 657 uppercase nucleotides (underlined) are the open reading frame of TNFRSF1A beta. The boxed uppercase letters represent the second set primers, while the boxed lowercase is the first set downstream primer. The underlined lowercase letters, "attaaa", are the putative polyadenylation signal. The lowercase poly(A) tail is also underlined. The nucleotide sequence of TNFRSF1A beta has been submitted to GenBank with the accession number EU927389 [GenBank:EU927389]. (B) Amino acid sequence alignment of the novel isoform of TNFRSF1A (TNFRSF1A beta) with TNFRSF1A RefSeq [GenBank:NP_001056]. The boxed region indicates the amino acid residues specific for TNFRSF1A beta. (C) The genomic structure and organization of functional domains of TNFRSF1A beta. Exons are numbered 1 to 10 at the top. Coding exons are represented by blocks connected by horizontal lines representing introns. The 5' and 3' untranslated regions (UTRs) are displayed as thinner blocks on the leading and trailing ends of the aligning regions. The 3'-end exon (grey block) for of TNFRSF1A beta is located between exon 5 and 6. The signal peptide (S), tumor necrosis factor receptor domain (TNFR), and transmembrane helix (TM) and death domain (DEATH) are indicated. Domain regions are numbered.