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Table 2 qRT-PCR validation of selected DEGs

From: A gene catalogue for post-diapause development of an anhydrobiotic arthropod Artemia franciscana

Gene

Expression Change*

 

ESTs

Q-RT-PCR

HSP26

-3.8

-5.08

Cystatin B inhibitor

-7

-2.06

LEA

-7.3

-7.10

cathepsin L-like protease

-3.4

-5.29

haloacid dehalogenase-like

-1.5

-3.55

HSP70

-11

-3.57

CDC48

0/5

+3.38

chaperonin-containing TCP

+3.33

+3.66

UCP2

+2.5

+10.55

GADPH

  
  1. * Fold changes are used for expression quantization; plus and minus indicate down- and up-regulations. For the EST data, we used transcripts per million (TPM) instead of absolute expression abundance, and for Q_PCR results, we calculated fold changes directly, according to the manufacture's instruction.
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BMC Genomics

ISSN: 1471-2164

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