Table 5 Primers used in the analysis of cell-wall-degrading enzymes
Primers | Sequence (5' to 3') | Use |
|---|---|---|
Eng1-0F | CTCTACGGGATGAAGTGTCT | In situ hybridization, amplification of cDNA and gDNA of Aa-eng-1 and Aa-eng-2 |
Eng1-0R | TTAACAAAAGCGGTACAAG | In situ hybridization, amplification of cDNA and gDNA of Aa-eng-1 and Aa-eng-2 |
Eng1-1F | GCTCAAGGTCGTCGTCGAGG | Sequencing of cDNA and gDNA of Aa-eng-1 and Aa-eng-2 |
Eng1-10F | GGCATCTCCGAGGCCGACG | Sequencing of gDNA of Aa-eng-1 and Aa-eng-2 |
Eng1-10R | CTTGCCGTACTCCTGCGCGAT | Sequencing of gDNA of Aa-eng-1 and Aa-eng-2 |
Eng2-20R | GCTACTTTGCTGGTCCACGT | Sequencing of gDNA of Aa-eng-2 |
Pel1-0F | TCCGACGACAACGTCAACCA | In situ hybridization, amplification of cDNA and gDNA of Aa-Pel-1 |
Pe11-0R | AAACCCTCAGCATGTTTGATAC | In situ hybridization, amplification of cDNA and gDNA of Aa-Pel-1 |
Pel1-1F | TCGAGAACGTCTGGTGGGA | Sequencing of cDNA and gDNA of Aa-Pel-1 and Aa-Pel-2 |
Pe11-10F | CTTGGAGGTACGCTTCGTACG | Sequencing of gDNA of Aa-Pel-1 |
Pe11-10R | TGACCTTCTTCGCCGCAGTG | Sequencing of gDNA of Aa-Pel-1 |
Pe11-20F | GAAATGGTACGATTAGTCCTG | Sequencing of gDNA of Aa-Pel-1 |
Pe12-0F | TCAGTCGGACAGCTTTTCCTC | Amplification of cDNA and gDNA of Aa-Pel-2 |
Pe12-0R | AGCAGGCATTTCGTCGACAC | Amplification of cDNA and gDNA of Aa-Pel-2 |
Pe12-10F | CGAGATGGCACGGGTGCCGA | Sequencing of gDNA of Aa-Pel-2 |
Pe12-10R | CGTAGCGAGAAATTTTCGATCA | Sequencing of gDNA of Aa-Pel-2 |