Figure 4From: De novo 454 sequencing of barcoded BAC pools for comprehensive gene survey and genome analysis in the complex genome of barleyEffect of different parameter settings on assemblies. Heat maps visualizing the assembly results of 454 sequences of the four complete reference BACs (top: set 1; bottom: set 2) by MIRA under different combinations of hss (hash saving steps, X-axis) and bph (bases per hash, Y-axis). BACs from left to right are: 184G09, 259I16, 631P08, 711N16. Black fields indicate the hss/bph combinations resulting in the largest contig for the respective BAC, dark to light gray fields mark values producing a contig with >90%, >50% and <50% of the largest contig length, respectively. White fields represent meaningless combinations (hss > bph).Back to article page