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Table 1 GTDs B transposition in F1 and F2 generation of gene trap lines

From: An Ac/Ds-mediated gene trap system for functional genomics in barley

Parental lines F1 generation F2 generation
GTDs B line GTDs B copies TPase linea No. plants analysed No. with tnpb F2 parent No. plants analysed No. with tnpb Independent tnpc No. pad
2a/d 1–2 2 3 - GT32 6 3 1 -
   4 9 - GT73 4 4 1 -
   7 1 - -     
23 1–2 2 4 - GT33 4 1 1 -
   3 2 1 GT29 4 4 3 -
   4 1 - GT39 1 1 1 1
   7 4 3 GT41 5 5 2 -
31B 2 4 4 - GT49 4 2 1 1
   7 5 2 GT54 5 3 1 -
6a/c 2–4 2 1 - GT70 4 - - -
   4 1 - GT67 7 - - 1
   7 2 - GT4 7 5 5 1
31A 4 2 2 - GT38 6 6 2 -
   4 1 - -     
   7 6 1 GT52 5 - - -
16 3–5 2 2 - GT19 4 3 1 -
   7 2 - GT37 7 2 2 -
   7 2 - GT40 4 4 2 -
14A 5–6 4 2 - GT13 4 3 1 1
   7 3 - GT22 5 - - 2
11 5–8 3 1 1 GT82 3 3 1 -
   4 1 - GT66 3 - - 1
   7 2 - GT80 54 16 10 3
10 6–7 2 4 - GT9 7 - - -
   4 3 - GT2 7 - - 5
   7 2 - GT6 7 1 1 -
14B 8–10 2 2 - GT23 4 2 2 -
   7 3 1 GT15 8 3 3 1
26 12 2 2 - GT35 4 3 3 1
   7 2 - GT63 8 5 5 3
Total    79 9   191 79 49 21
  1. aTPase line 3 and 4 carry four and two copies of the TPase construct respectively, whereas 2 and 7 carry one and four TPase103–807 constructs respectively
  2. bNumber of plants showing GTDs B transposition (tnp)
  3. cNumber of plants with independent transposition events
  4. dNumber of plants with phenotypic abnormality (pa)