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Table 1 GTDs B transposition in F1 and F2 generation of gene trap lines

From: An Ac/Ds-mediated gene trap system for functional genomics in barley

Parental lines

F1 generation

F2 generation

GTDs B line

GTDs B copies

TPase linea

No. plants analysed

No. with tnpb

F2 parent

No. plants analysed

No. with tnpb

Independent tnpc

No. pad

2a/d

1–2

2

3

-

GT32

6

3

1

-

  

4

9

-

GT73

4

4

1

-

  

7

1

-

-

    

23

1–2

2

4

-

GT33

4

1

1

-

  

3

2

1

GT29

4

4

3

-

  

4

1

-

GT39

1

1

1

1

  

7

4

3

GT41

5

5

2

-

31B

2

4

4

-

GT49

4

2

1

1

  

7

5

2

GT54

5

3

1

-

6a/c

2–4

2

1

-

GT70

4

-

-

-

  

4

1

-

GT67

7

-

-

1

  

7

2

-

GT4

7

5

5

1

31A

4

2

2

-

GT38

6

6

2

-

  

4

1

-

-

    
  

7

6

1

GT52

5

-

-

-

16

3–5

2

2

-

GT19

4

3

1

-

  

7

2

-

GT37

7

2

2

-

  

7

2

-

GT40

4

4

2

-

14A

5–6

4

2

-

GT13

4

3

1

1

  

7

3

-

GT22

5

-

-

2

11

5–8

3

1

1

GT82

3

3

1

-

  

4

1

-

GT66

3

-

-

1

  

7

2

-

GT80

54

16

10

3

10

6–7

2

4

-

GT9

7

-

-

-

  

4

3

-

GT2

7

-

-

5

  

7

2

-

GT6

7

1

1

-

14B

8–10

2

2

-

GT23

4

2

2

-

  

7

3

1

GT15

8

3

3

1

26

12

2

2

-

GT35

4

3

3

1

  

7

2

-

GT63

8

5

5

3

Total

  

79

9

 

191

79

49

21

  1. aTPase line 3 and 4 carry four and two copies of the TPase construct respectively, whereas 2 and 7 carry one and four TPase103–807 constructs respectively
  2. bNumber of plants showing GTDs B transposition (tnp)
  3. cNumber of plants with independent transposition events
  4. dNumber of plants with phenotypic abnormality (pa)
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BMC Genomics

ISSN: 1471-2164

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