The salivary basic tail family of mosquito proteins. (A) Clustal alignment. The sole An. darlingi sequence is identified by AD-217. The remaining sequences are named with the first three letters from the genus name followed by two letters from the species name and by their NCBI protein accession number. Conserved cysteines are shown in black, hydrophobic conserved amino acids (aa) in light blue, conserved Pro and Gly in yellow, conserved bulky non-charged aa (Asn, Gln, Ser, Thr) in grey, conserved Ser + Thr in brown, conserved negatively charged aa in red, identical positively charged aa in violet, conserved charged aa in green. The symbols above the alignment indicate: (*) identical sites; (:) conserved sites; (.) less conserved sites. (B) Phylogram derived from the alignment in (A). The numbers on the tree nodes represent the percent bootstrap support in 10,000 trials (only values above 50% are shown). The bar at the bottom indicates 10% aa divergence.