Clustal alignment of the 41.9-kDa family of mosquito proteins. The sole An. darlingi sequence is identified by AD-114. The remaining sequences are named with the first three letters from the genus name followed by two letters from the species name and by their NCBI protein accession number. For more details, see text. Conserved cysteines are shown in black, hydrophobic conserved amino acids (aa) in light blue, conserved Pro and Gly in yellow, conserved bulky non-charged aa (Asn, Gln, Ser, Thr) in grey, conserved Ser + Thr in brown, conserved negatively charged aa in red, identical positively charged aa in violet, conserved charged aa in green. The symbols above the alignment indicate: (*) identical sites; (:) conserved sites; (.) less conserved sites.