Figure 7
MiR-N1, miR-N2, and miR-N3 expression is restricted in particular lineages in mosquitoes. A) miR-N1 is expressed in Ae. aegypti and Cx. quinquefasciatus, but not in An. stephensi nor T. amboinensis. Emb, pooled embryos between 0-36 hr after egg deposition; L1+2, pooled 1st and 2nd instar larvae; L3+4, pooled 3rd and 4th instar larvae; Pupae, mixed pupae; F, adult females one to five days after emergence; M, adult males one to five days after emergence. Culex Emb, Cx. quinquefasciatus embryos 0-24 hrs after egg deposition. "+ Cont", positive control, Ae. aegypti embryos (12-24 hr). 15 μg of total RNA per sample for An. stephensi and Ae. aegypti were used. 10 μg of T. amboinensis total RNA per sample were used. For T. amboinensis, 3rd instar larvae were not included. B) miR-N2 is expressed in Ae. aegypti but not detected in Cx. quinquefasciatus and An. stephensi embryos. Symbols are as in A. Anopheles Emb, pooled An. stephensi embryos between 0-36 hr after egg deposition. C) miR-N3 is expressed in Cx. quinquefasciatus, but not detected in Ae. aegypti and An. stephensi embryos. Symbols are as in A and B. Aedes Emb, pooled Aedes aegypti embryos between 0-36 hr after egg deposition.