The transcriptional programme of Salmonella enterica serovar Typhimurium reveals a key role for tryptophan metabolism in biofilms

Table 4 Bacterial strains and plasmids.

Strain/plasmid	Relevant characteristics	Reference/source
*S. enterica* serovar Typhimurium strains
SL1344	WT (rpsL hisG) mouse virulent	[109, 110]
JH3182	SL1344/ΔSTM2779:: Km	This study
JH3183	SL1344 ΔygaT:: Km	This study
JH3184	SL1344 ΔybaY:: Km	This study
JH3185	SL1344 ΔtrpE:: Km	This study
JH3186	SL1344 ΔyhfG:: Km	This study
JH3187	SL1344 ΔSTM0341:: Km^$	This study
JH3188	SL1344 Δmtr:: Km	This study
JH3189	SL1344 ΔpspA-E:: Km	This study
JH3142	SL1344 ΔrpoS:: Amp	Laboratory collection
JH3180	SL1344 ssrA::mTn5(Km^r)*	This study
JH3181	SL1344 ΔssrA(pSsrAB)	This study
JH3179	SL1344 Δorf319::Cm	This study
LT2A	WT lab strain	[100]
JH4000	LT2A Δhns	[111]
Plasmids
pKD4	Template plasmid, Km^R	[103]
pKD46	Lambda Red helper plasmid, Amp^R	[103]
pWSK29	pssrAB	[58]
p1437-1	bla pBAD-ssrAB ⁺	K. Tedin
p1437-6	bla pBAD-ssrAB ^-	K. Tedin

* The ssrA::mTn5 mutation [111] was transduced to SL1344 from strain P3F4 (12023 ssrA::mTn5).
^$ It should be noted that the polar mutation in STM0341 could affect expression of the downstream STM0342 gene; both of these genes are highly down-regulated during biofilm growth. Therefore, the putative periplasmic protein encoded by STM0342 may play a role in the phenotypes associated with strain JH3187.

ISSN: 1471-2164