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Table 1 Proteins in the epidermis during metamorphosis identified by Mascot searches using protein mass fingerprints (PMF) and MS/MS against the NCBInr_Metazoa Database

From: Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera

  

Description

PMF

MS/MS

  

Spot

Protein name

GenBank Accession number and organism

Theoretical MW(kD)/pI

Experimental MW(kD)/pI

Peptide hits

Score*

Coverage

Peptide hits

Score*

Peptide sequences

A

P

22

viral-like chitinase

gi|74422655

[Spodoptera exigua]

61.6/6.14

64/6.55

27

211

38%

-

-

-

↑

-

27

hexamerin

gi|50404096

[Helicoverpa armigera]

82.0/5.89

75/6.15

17

129

23%

-

-

-

↑

-

28

hexamerin

Gi|50404096

[Helicoverpa armigera]

82.0/5.89

75/6.21

23

159

26%

-

-

-

↑

-

29

hexamerin

Gi|50404096

[Helicoverpa armigera]

82.0/5.89

75/6.25

25

136

32%

-

-

-

↑

-

36

prophenoloxidase-1

gi|159141810

[Heliothis virescens]

79.1/6.39

67/5.95

11

97

20%

-

-

-

↑

↑

37

arylphorin subunit

gi|5869989

[Spodoptera litura]

76.8/6.7

56/6.75

8

71

12%

2

   55

RDPAFYQLYQRI

KDLHQYSYEIIARH

↑

↑

40

heat shock protein 1

gi|56462154

[Lonomia obliqua]

21.9/6.32

16/4.95

9

111

38%

4

   230

RNSYFRPWRT

RLLDQHFGMGLKR

RDWWDEWDRPSRL

RHEEKQDEHGFISRQ

↑

↑

42

Phosphoglyceromu-tase

gi|1092224

[Drosophila melanogaster]

28.6/6.62

28/6.1

-

-

-

2

   98

RILIAAHGNSLRG

KYGEAQVQIWRR

↑

↑

44

aldehyde

dehydrogenase

gi|164450344

[Ectropis obliqua]

22.0/5.67

(partial)

72/5.89

-

-

-

1

   57

KFETFEEVVDRA

↑

↑

45

solute carrier family 25

gi|123232765

[Mus musculus]

55.0/8.81

67/5.55

12

77

24%

-

-

-

↑

↑

46

fructose 1,6-bisphosphate aldolase

gi|45330818

[Antheraea yamama i]

39.7/7.59

44/7.39

12

123

37%

-

-

-

↑

↑

50

large subunit arylphorin p76

gi|27802137

[Heliothis virescens]

30.6/6.33

(partial)

72/6.32

9

91

35%

-

-

-

↑

↑

52

60 S acidic ribosomal

protein P0

gi|18253041

[Spodoptera frugiperda]

34.0/6.34

37/6.35

11

168

53%

-

-

-

↑

↑

55

calponin/transgelin

gi|108875705

[Aedes aegypti]

19.2/7.77

18/7.75

9

121

43%

1

   61

KYGVPEEEIFQTADLFERR

↑

↑

58

eukaryotic translation

initiation factor 4A

gi|95102876

[Bombyx mori]

48.0/5.1

53/5.26

29

238

53%

-

-

-

No

↓

59

ATP synthase

beta subunit

gi|115345328

[Bombyx mori]

54.9/5.32

54/5.05

25

236

55%

5

   211

RIINVIGEPIDERG

KAHGGYSVFAGVGERT

RVALTGLTVAEYFRD

KVSLVYGQMNEPPGARA

RLVLEVAQHLGENTVRT

No

↓

60

proteasome alpha 3 subunit

gi|114051245

[Bombyx mori]

28. 4/5.27

31/5.3

11

100

30%

2

   67

KLYEPGANKRI

KAVENSGTVIGLRG

No

↓

61

heat shock protein 1

gi|56462154

[Lonomia obliqua]

21.9/6.32

27/5.75

7

90

27%

-

-

-

No

↑

  1. Symbols in the table: *, PMF score > 74 significant, MS/MS score > 45 significant; A, protein variation; P, phosphorylation variation; -, not known; ↑, increase compared to 5th-F; ↓ decrease compared to 5th-F; No, no change.
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BMC Genomics

ISSN: 1471-2164

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