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Table 1 Proteins in the epidermis during metamorphosis identified by Mascot searches using protein mass fingerprints (PMF) and MS/MS against the NCBInr_Metazoa Database

From: Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera

   Description PMF MS/MS   
Spot Protein name GenBank Accession number and organism Theoretical MW(kD)/pI Experimental MW(kD)/pI Peptide hits Score* Coverage Peptide hits Score* Peptide sequences A P
22 viral-like chitinase gi|74422655
[Spodoptera exigua]
61.6/6.14 64/6.55 27 211 38% - - - -
27 hexamerin gi|50404096
[Helicoverpa armigera]
82.0/5.89 75/6.15 17 129 23% - - - -
28 hexamerin Gi|50404096
[Helicoverpa armigera]
82.0/5.89 75/6.21 23 159 26% - - - -
29 hexamerin Gi|50404096
[Helicoverpa armigera]
82.0/5.89 75/6.25 25 136 32% - - - -
36 prophenoloxidase-1 gi|159141810
[Heliothis virescens]
79.1/6.39 67/5.95 11 97 20% - - -
37 arylphorin subunit gi|5869989
[Spodoptera litura]
76.8/6.7 56/6.75 8 71 12% 2    55 RDPAFYQLYQRI
KDLHQYSYEIIARH
40 heat shock protein 1 gi|56462154
[Lonomia obliqua]
21.9/6.32 16/4.95 9 111 38% 4    230 RNSYFRPWRT
RLLDQHFGMGLKR
RDWWDEWDRPSRL
RHEEKQDEHGFISRQ
42 Phosphoglyceromu-tase gi|1092224
[Drosophila melanogaster]
28.6/6.62 28/6.1 - - - 2    98 RILIAAHGNSLRG
KYGEAQVQIWRR
44 aldehyde
dehydrogenase
gi|164450344
[Ectropis obliqua]
22.0/5.67
(partial)
72/5.89 - - - 1    57 KFETFEEVVDRA
45 solute carrier family 25 gi|123232765
[Mus musculus]
55.0/8.81 67/5.55 12 77 24% - - -
46 fructose 1,6-bisphosphate aldolase gi|45330818
[Antheraea yamama i]
39.7/7.59 44/7.39 12 123 37% - - -
50 large subunit arylphorin p76 gi|27802137
[Heliothis virescens]
30.6/6.33
(partial)
72/6.32 9 91 35% - - -
52 60 S acidic ribosomal
protein P0
gi|18253041
[Spodoptera frugiperda]
34.0/6.34 37/6.35 11 168 53% - - -
55 calponin/transgelin gi|108875705
[Aedes aegypti]
19.2/7.77 18/7.75 9 121 43% 1    61 KYGVPEEEIFQTADLFERR
58 eukaryotic translation
initiation factor 4A
gi|95102876
[Bombyx mori]
48.0/5.1 53/5.26 29 238 53% - - - No
59 ATP synthase
beta subunit
gi|115345328
[Bombyx mori]
54.9/5.32 54/5.05 25 236 55% 5    211 RIINVIGEPIDERG
KAHGGYSVFAGVGERT
RVALTGLTVAEYFRD
KVSLVYGQMNEPPGARA
RLVLEVAQHLGENTVRT
No
60 proteasome alpha 3 subunit gi|114051245
[Bombyx mori]
28. 4/5.27 31/5.3 11 100 30% 2    67 KLYEPGANKRI
KAVENSGTVIGLRG
No
61 heat shock protein 1 gi|56462154
[Lonomia obliqua]
21.9/6.32 27/5.75 7 90 27% - - - No
  1. Symbols in the table: *, PMF score > 74 significant, MS/MS score > 45 significant; A, protein variation; P, phosphorylation variation; -, not known; ↑, increase compared to 5th-F; ↓ decrease compared to 5th-F; No, no change.