Table 3 List of oligonucleotide primers used for RT, PCR and RACE-PCR experiments.
GENE | ORF NUMBER | PRIMER NAME | SEQUENCE (5'-3') | POSITION ON CHROMOSOME SEQUENCE |
|---|---|---|---|---|
unk | ECU02_0700 | 5'-unk | ATGCCATCTCGGCACTTAGAAGGCTC | complement (86499..86524) |
3'-unk | GCGCTCCAGCTCATCAGCGGCGAG *# | 86443..86466 | ||
htr | ECU02_0710 | 5'-htr | ACCTGTCCGTCCATACCACGTTGC | 88604..88627 |
3'-htr | GATACCGACCTGGCGCTTGAACACG # | complement (87230..87254) | ||
h2a | ECU02_0720 | 5'-h2a | TGATCTCGCTGATTAGGTACATTAC | 89217..89241 |
3'-h2a | GGACCAGGATGAGGATATCGAAGG | complement (89268..89291) | ||
pap | ECU02_0730 | 5'-pap | TCAAAGAACCCACGCTCCTGTAGG | 90874..90897 |
3'-pap | GTCAAAGTTCGAGGCGGTTGACGAC | complement (89812..89836) | ||
ubi | ECU02_0740i | 5'ubi | CTCGATACTGTCCGAAGGCTCGACT # | 91300..91324 |
3'-ubi | AGTCGAGCCTTCGGACAGTATCGAG | complement (91300..91324) | ||
rib | ECU02_0750i | 5'-rib | TGTCATGCTCTGACAGAACCGTCC *# | complement (91758..91781) |
3'-rib | AGAAGAAGGTATCCAAGCTGGCC | 91698..91720 |