Linking mechanistic and behavioral responses to sublethal esfenvalerate exposure in the endangered delta smelt; Hypomesus transpacificus (Fam. Osmeridae)

Table 4 Primers and probes used for molecular biomarkers: Primer pairs and TaqMan probes used in q-PCR assessments.

Accession no.	Gene		Primer Sequences	Probe No.
FJ711577	Aspartoacylase	Left:	ggaggcacacatgggaatg	109
		Right:	cttcctctgaatctctgttccattatc
FJ711576	Parvalbumin	Left:	gaccaagacaagagtggcttca	101
		Right:	tctggcaccagcagagaagtt
FJ711580	β-2 microglobulin	Left:	tctttcgcggtcatctttctc	22
		Right:	ggttgtggccatacacctgaa
FJ711579	Hemopexin	Left:	catgcactacgaggacgacaag	143
		Right:	tggtagtagctgaacaccttgctg
FJ711578	Caspase-3	Left:	gagaaccggtatgaaccaacg	159
		Right:	tccaagcttcccaaacactttc
FJ711575	Titin a	Left:	tgatcactggcgtgaaagagg	159
		Right:	caagctcattggacagtttgagg
FJ711581	ZPA	Left:	catgcggctgagtttggataa	106
		Right:	tgccattgatagcatcaacttca
FJ711583	Myozenin*	Left:	ccaatgtcgtgctggtacacc	106
		Right:	ctgccagacattgatgtagcca
FJ711584	Creatine kinase*	Left:	cgatcggcgttggagatg	163
		Right:	gccaagttcaacgagattctgg
FJ711582	PXR	Left:	tgaggcggtggagaagag	144
		Right:	gaggcggtggagaagag

* indicates biomarkers obtained from preliminary studies carried out during microarray development.

ISSN: 1471-2164