Understanding Haemophilus parasuis infection in porcine spleen through a transcriptomics approach

BMC Genomics

Table 1 Primers used for QPCR evaluation on cell migrations

Gene	Marker for cell types	Primer sequence (5'-3')	Target size (bp)	Tm (°C)^a
CD197	T cell dendritic cell	Forward: TGTGGTTTCAGCAGCCAAGAG Reverse: GCCGATGTAGTCGTCCGTGA	130	59.8
CD11B	Macrophage Granulocyte	Forward: CAACCTGGGTCAGAGGAAGC Reverse: CAGACAGCGATGGAGCAGTT	207	62
CD3E	T cell	Forward: ACCTCTTAGTTCCTCCCTTTG Reverse: TGCCAGCATTTACCCAGTC	137	59.8
CD14	Macrophage Granulocyte	Forward: GCAGAGGCTTTGAGGACCTTATC Reverse: GCTGCGGATGCGTGAAGTT	154	62
CD4	T cell NK cell	Forward: TCTGCGAAGTGGAAGACAAG Reverse: GCTCTTGACGTCATTCTTGC	179	59.8
RPL32 ^b		Forward: CGGAAGTTTCTGGTACACAATGTAA Reverse: TGGAAGAGACGTTGTGAGCAA	94	59.8–62

^aThe annealing temperature represents the optimal temperature during quantitative PCR. ^bRNA levels of RPL32 was assayed for normalization during quantitative PCR.

ISSN: 1471-2164