Table 3 Primers used for QPCR validation and additional expression profiling
From: Understanding Haemophilus parasuis infection in porcine spleen through a transcriptomics approach
Gene | Primer sequence (5'-3') | Target size (bp) | Tm (°C)a |
|---|---|---|---|
RETN | Forward: CCTCTTCCTCCCAACCCTG Reverse: GAGGTGACACTCCGGCATT | 150 | 62 |
S100A12 | Forward: GGCATTATGACACCCTTATC Reverse: GTCACCAGGACCACGAAT | 168 | 60 |
S100A9 | Forward: CCAGGATGTGGTTTATGGCTTTC Reverse: CGGACCAAATGTCGCAGA | 186 | 60 |
HIRF b | Forward: TTACCAGTGCCTCTCCTCCAT Reverse: GCGTGACAAACCCCAGTTATCT | 127 | 60 |
CFP | Forward: TTCCCGCCCACCATTACC Reverse: GCCGTTTCTCCTCCACCATC | 121 | 60 |
HP | Forward: ACTCCACGGTAGACATCGG Reverse: GTTGAGGTTGGCGTTTCG | 149 | 63 |
S100A8 | Forward: GCGTAGATGGCGTGGTAA Reverse: GCCCTGCATGTGCTTTGT | 155 | 60 |
THY1 | Forward: CGGCTATTCGTCCTCTTTGTT Reverse: CCGACCCTGGCTTCCCTT | 102 | 60 |
CCL5 | Forward: CCTGAGACAGCCCGTGGAT Reverse: GGTGTGGTGTCCGAGGCAT | 141 | 60 |
ERAF | Forward: GCCCTTCTTCCAACCAATCA Reverse: CCCCCACCATCTTCTTCTTGTAG | 172 | 60 |
RPL32 c | Forward: CGGAAGTTTCTGGTACACAATGTAA Reverse: TGGAAGAGACGTTGTGAGCAA | 94 | 60–63 |