Figure 6

The whole genome sequence of a representative CBSN isolate of emergent multi-drug resistant serotype 19A ST320 (Genbank Accession GPID ACNU00000000) was compared to a representative isolate of 19F ST320 from the pre-vaccine era (Genbank Accession GPID ACNV00000000). The Solexa platform (Illumina Inc, San Diego, CA) was used for sequencing with greater than 100X coverage obtained throughout each genome. Depicted here is the whole genome of a representative MDR 19A ST320 in the post-vaccine era. The locations of proteins encoded on the leading and lagging strands are shown on the outer two rings. Gene ontology categories are color-coded. Of the internal ring, the outermost bars indicate SNPs identified in the leading strand and the innermost bars represent SNP identified in the lagging strand relative to MDR 19F ST320. The length of the bars is propostional to the number of SNPs. For detailed gene identification, location of capsule biosynthetic loci, other key alleles, comparison of 19A and 19F SNPs, as well as a mutation profile compared to reference strain R6 (Genbank AE007317), see Additional file 4http://www.pillailab.com/suppdata/index.html.