Table 1 Real-time PCR primer combinations used to amplify genes of interest.
NCBI accession (cDNA clone) | NCBI accession for mRNA record | Primer | Sequence of primer | Position | Size (bp) |
|---|---|---|---|---|---|
AI044043 | NM_001009172.2 | ZBTB22 (F) | 5'-GAGAGCCCGAGGCTACCCT-3' | 1204 – 1222 | 74 |
|  |  | ZBTB22 (R) | 5'-CCTGTTCCTCCCCCTTTTCT-3' | 1278 – 1259 |  |
AI059162 | NM_022605.1 | HPSE (F) | 5'-GGCTCTCCACGGCTTCG-3' | 270 – 286 | 65 |
|  |  | HPSE (R) | 5'-GGTGCCGCCAAATCTCAA-3' | 335 – 318 |  |
AI145841 | NM_199267.2 | RelA (F) | 5'-GGCATGCGTTTCCGTTACA-3' | 152 – 170 | 68 |
|  |  | RelA (R) | 5'-ATCTGTGCTTCTCTCCCCAGG-3' | 220 – 200 |  |
AI058327 | XM_576071.1 | MLK1 (F) | 5'-ACCCCAGCTGCTACCCG-3' | 359 – 375 | 71 |
|  |  | MLK1 (R) | 5'-CGATGATCTCCTCCAGGGTTAG-3' | 430 – 409 |  |
AA924727 | Z78279.1 | COL1A1 (F) | 5'-AAAACGGGAGGGCGAGTG-3' | 228 – 245 | 80 |
|  |  | COL1A1 (R) | 5'-GGTCCCTCGACTCCTATGACTTC-3' | 308 – 286 |  |
AI145995 | AY648296.1 | PPP1R1A (F) | 5'-AGACTCAGGAGCAGCGTGGT-3' | 559 – 578 | 65 |
|  |  | PPP1R1A (R) | 5'-CCAGTGGTAGCATGTGGGC-3' | 624 – 606 |  |
AI044118 | NM_001107425.1 | NFAT5 (F) | 5'-CCTTTAAGGATGCGCTCCAA-3' | 13098 – 13117 | 77 |
|  |  | NFAT5 (R) | 5'-CAGGGAGGAAAAGAAGAGAGTGAA-3' | 13151 – 13174 |  |