Comparative analysis of two Neisseria gonorrhoeae genome sequences reveals evidence of mobilization of Correia Repeat Enclosed Elements and their role in regulation

Table 1 CREE 5' of important gonococcal genes

Gene product	FA1090*	NCCP11945†	CREE distance‡
Drg restriction endonuclease¶	NGO0007	NGK_0010	22 bp
TonB-dependent receptor	NGO0021	NGK_0029	36 bp
MafB adhesion	NGO0225	NGK_0360	33 bp
UvrB excinuclease subunit**	NGO0573	NGK_1349	124 bp
MarR regulator††	NGO1244	NGK_0263	34 bp
LpxB lipid A biosynthetic pathway protein	NGO1782	NGK_2492	‡‡
HpuA haemoglobin utilization protein	¶¶	NGK_2581	138 bp
Cadmium resistance protein	NGO2127	NGK_2601***	66 bp
RegF pilin regulator	NGO2130	NGK_2604	42 bp
ComE competence protein	NGO1178	NGK_1527	47 bp
	NGO1304	NGK_1966
	NGO1902	NGK_2096

* Locus annotation from N. gonorrhoeae strain FA1090 [GenBank: RefSeq NC_002946].
† Locus annotation from N. gonorrhoeae strain NCCP11945 [GenBank: RefSeq NC_011035].
‡ Distance the CREE is from the initiation codon of the gene.
¶As reported previously [12].
** As reported previously [10].
†† Both types of CREE-associated promoters [10, 11] are present. This regulator is specific to the pathogenic Neisseria spp. and controls different regulons in N. gonorrhoeae and N. meningitidis [34].
‡‡ The A of the ATG initiation codon is the final A of the CREE.
¶¶ Unannotated in strain FA1090 where the CDS is frame-shifted at a poly-G tract.
*** This CDS is frame-shifted in strain NCCP11945.

ISSN: 1471-2164