Skip to main content

Table 1 Strain construction

From: Exploring and dissecting genome-wide gene expression responses of Penicillium chrysogenum to phenylacetic acid consumption and penicillinG production

ID primer Gene Direction Sequence
1 pcbC FWD GAT TGG CGC TCC TCG TTC ACC
2 pcbC REV CCA TTA TTT TTC TAG TCG ACA TGG CAT CGA TTC CCA AGG CCA ATG TCC CC
5 LF 7-kb FWD GTT ACA CGC TTT GAT TCT GTG GGT ACC GAT GTT ATA TTC AGC TAC
6 LF REV CCC AAT AGC GGC CGC AGT TGA TAA TAT CAA TAT CTA AAA CTC CC
7 LF 5-kb FWD GGC ATA TAC GAG CAT GGT ACC AGG GAC AGA TGC CCA TCC TTG
8 LF 3-kb FWD GTA TAA AAG GGG AGG GTA CC G GGA AAG ATT TGT GGG CCT G
9 RF FWD GTA TGT AGC TGC GGC CGC C TC CGT CTT CAC TTC TTC GCC CGC ACT
10 RF 3-kb REV CCG CCT TCC TCA CTA ACC GGC CGG CAG GTA CC G ATG GAC TCA GCA TTA TC
11 RF 5-kb REV CTC TAG AAT GCT ACG GCC GTT CGA GGT ACC TTA TAG GAA AAA GGT AG
12 RF 7-kb REV CCT TTT CGC TGA GCG GCC GCA ATC ACA GGT ACC GTT TTT GTC GTC
13 amdS FWD ATG CCT CAA TCC TGG GAA GAA CTG
14 amdS REV CTT GAC GTA GAA GAC GGC ACC GGC
15 penDE FWD CCC GCA GCA CAT ATG CTT CAC ATC CTC TGT CAA GGC
16 penDE REV ATG ACA AAC ATC TCA TCA GGG
17 niaA FWD CAC AGA GAA TGT GCC GTT TCT TTG G
18 niaA REV TCA CAT ATC CCC TAC TCC CGA GCC
19 pcbAB FWD GAA GAC GTC ATA CTT ATT CTC TG
20 pcbAB REV CGG CAT CGG ATA AAG AGA TCT GG
  1. Primers used in this study (restriction sites are in bold). LF and RF, Left and Right flanking region respectively, location of the flanking region relative to the amdS-cassette (see Figure 1A). Size of the flanking region in kb. ID primer, uniquely numbered identifier of the primer used.